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Site-directed point mutations in embryonic stem cells: a gene-targeting tag-and-exchange strategy

Published in: Molecular and Cellular Biology

Date of publication: July 1993

Language: English

Bibliographic databases:

Cites articles 28

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The effects of erythropoietin on haem synthesis in mouse yolk sac and cultured foetal liver cells
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Rapid and efficient site-specific mutagenesis without phenotypic selection
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Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination

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The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium
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Transgenesis by means of blastocyst-derived embryonic stem cell lines
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Structure-function relationships in the Na,K-ATPase alpha subunit: site-directed mutagenesis of glutamine-111 to arginine and asparagine-122 to aspartic acid generates a ouabain-resistant enzyme
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Potential for genetic manipulation of mammals
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Production of chimaeric mice containing embryonic stem (ES) cells carrying a homoeobox Hox 1.1 allele mutated by homologous recombination
September 21, 1990

The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain
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Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development
December 1, 1990

Gene targeting in murine embryonic stem cells: introduction of specific alterations into the mammalian genome
April 1, 1990

Beta 2-microglobulin deficient mice lack CD4-8+ cytolytic T cells
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Infertility in male transgenic mice: disruption of sperm development by HSV-tk expression in postmeiotic germ cells
February 1, 1991

Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice
August 11, 1991

Simplified mammalian DNA isolation procedure
March 1, 1991

Testing an "in-out" targeting procedure for making subtle genomic modifications in mouse embryonic stem cells
September 1, 1991

Fidelity of targeted recombination in human fibroblasts and murine embryonic stem cells
May 1, 1991

Cotransformation and gene targeting in mouse embryonic stem cells
July 1, 1991

Isolation of digoxin-like immunoreactive factors from mammalian adrenal cortex
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Introduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells

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High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors
October 22, 1992

Targeted disruption of the mouse transforming growth factor-beta 1 gene results in multifocal inflammatory disease
August 1, 1992

Effects of mutation position on frequency of marker rescue by homologous recombination
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Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs
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Investigation of coelectroporation as a method for introducing small mutations into embryonic stem cells
August 1, 1992

Targeting of the creatine kinase M gene in embryonic stem cells using isogenic and nonisogenic vectors
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Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus,Reexamination of Gene Targeting Frequency as a Function of the Extent of Homology between the Targeting Vector and the Target Locus
July 1, 1992

Disruption of the cystic fibrosis transmembrane conductance regulator gene in embryonic stem cells by gene targeting

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