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Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

Author

Douglas A. Melton

1953 -

Published in: Nucleic Acids Research

Date of publication: September 25, 1984

Language: English

Main subject: bacteriophage, genetics

Bibliographic databases:

Cites articles 38

Date

Title

Authors

December 1, 1969

Termination Factor for RNA Synthesis
June 1, 1972

RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed?
May 1, 1975

Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system
June 1, 1975

Termination of transcription in bacteriophage lambda. Heterogeneous, 3'-terminal oligo-adenylate additions and the effects of rho factor
February 1977

A new method for sequencing DNA
1977

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide
November 1977

Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
January 1, 1977

Removal of RNase activity from DNase by affinity chromatography on agarose coupled aminophenylphosphoryl-uridine-2' (3')-phosphate
December 1, 1978

Detection of viral sequences of low reiteration frequency by in situ hybridization
January 1, 1979

Extracellular nucleases of Alteromonas espejiana BAL 31.IV. The single strand-specific deoxyriboendonuclease activity as a probe for regions of altered secondary structure in negatively and positively supercoiled closed circular DNA
November 1, 1979

Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinates
September 1, 1979

Splicing of yeast tRNA precursors: a two-stage reaction
June 1, 1979

Efficient translation of prokaryotic mRNAs in a eukaryotic cell-free system requires addition of a cap structure
July 1, 1980

DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract
October 1, 1980

Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene
January 1, 1980

Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping
November 1, 1980

Definition of a novel promoter for the major adenovirus-associated virus mRNA.
June 1, 1981

Detection of poly A+ RNA in sea urchin eggs and embryos by quantitative in situ hybridization,Detection of poly A+RNA in sea urchin eggs and embryos by quantitative in situ hybridization
June 1, 1981

Transcripts from both strands of a satellite DNA occur on lampbrush chromosome loops of the newt Notophthalmus
December 1, 1981

Utilization of bacteriophage T7 late promoters in recombinant plasmids during infection
November 1982

Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena,Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena
October 1982

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments
May 1, 1982

Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme
May 1, 1982

Bacteriophage SP6-specific RNA polymerase. II. Mapping of SP6 DNA and selective in vitro transcription
September 1, 1983

Splicing of adenovirus RNA in a cell-free transcription system
December 1, 1983

Autocatalytic replication of a recombinant RNA.
October 1, 1983

Identification of two distinct regulatory regions adjacent to the human β-interferon gene
March 1, 1983

Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei
June 1, 1983

The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro

,
June 1, 1983

Accurate and specific polyadenylation of mRNA precursors in a soluble whole-cell lysate
November 1, 1983

Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts
April 1984

Genomic sequencing

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