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Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents

Author

Frances Arnold

Published in: Nature Biotechnology

Date of publication: April 1, 1996

Language: English

Main subject: directed evolution

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Cites articles 20

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Authors

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De-esterification of cephalosporin para-nitrobenzyl esters by microbial enzymes
March 1, 1990

Membrane-enclosed crystals in Dictyostelium discoideum cells, consisting of developmentally regulated proteins with sequence similarities to known esterases
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Complete sequence of rabbit butyrylcholinesterase
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DNA polymerase fidelity and the polymerase chain reaction
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Randomization of genes by PCR mutagenesis
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Purification and properties of an Arthrobacter oxydans P52 carbamate hydrolase specific for the herbicide phenmedipham and nucleotide sequence of the corresponding gene
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1.8 A refined structure of the lipase from Geotrichum candidum
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Comparative protein modelling by satisfaction of spatial restraints
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Cloning and analysis of Candida cylindracea lipase sequences

, , ,
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Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide
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The Bacillus subtilis pnbA gene encoding p-nitrobenzyl esterase: cloning, sequence and high-level expression in Escherichia coli
August 4, 1994

Rapid evolution of a protein in vitro by DNA shuffling
October 25, 1994

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution
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Derivation of rules for comparative protein modeling from a database of protein structure alignments
October 1, 1994

Acetylcholinesterase and butyrylcholinesterase expression in adult rabbit tissues and during development
January 1, 1996

Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide
December 1996

Directed evolution: Creating biocatalysts for the future
March 1, 1996

Improved green fluorescent protein by molecular evolution using DNA shuffling,Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling

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